Hazard ratios for patients with fIGF-1 values above or below the 0.5 0.9 ng ml−1 criteria are shown in Figure 2A.
Pre-specified acceptance criteria were set for the average within RNA input standard deviation pooled between 20 ng, 10 ng and 5 ng RNA input (precision) and the within and between RNA input (20 ng, 10 ng and 5 ng) standard deviation (reproducibility).
The percentages of patients who fulfilled criteria for NG, preDM, and DM according to FG or HbA1c criteria were calculated at baseline and follow-up.
Indeed, with the emergence of the ASTRO Phoenix criteria (nadir+2 ng ml−1) (Roach et al, 2006), the old definition is in itself questionable.
Samples that meet the following criteria: >50 ng RNA with absorbance ratios A260/280 and A260/230 of ~2.0 and RIN (RNA Integrity Number) > 7.5, were used for further analyses.
Samples meeting the quality criteria (≥200 ng total RNA, RIN ≥ 7 and 28S/18S ≥ 1) were shipped to the Beijing Genomics Institute (BGI) in dry ice for RNA-sequencing.
After 1 year, three of the eleven B-FG patients (28%) reversed to NG, four patients (36%) fulfilled criteria for preDM (FG only: n=3, HbA1c only: n=1), and four patients (36%) still fulfilled criteria for DM (FG only: n=4).
At baseline, 90 out of 169 patients (53%) were identified with NG, whereas 66 (39%) fulfilled criteria for preDM (FG only: n=32, HbA1c only: n=21, both: n=13), and 13 (8%) were diagnosed with DM (FG only: n=11, HbA1c only: n=1, both: n=1).
For PCF20 vs PC, the estimated treatment biomarker interaction terms from Cox proportional hazards models were between 2.5 and 3.5 for fIGF-1 criteria in the 0.5 0.9 ng ml−1 range, with one-sided P-values of 0.11 (0.5 ng ml−1 criterion), 0.09 (0.6 ng ml−1 criterion), 0.030 (0.7 ng ml−1 criterion), 0.033 (0.8 ng ml−1 criterion) and 0.026 (0.9 ng ml−1 criterion) adjusted for multiple testing.
Cox proportional hazards model interactions were between 2.5 and 3.5 for fIGF-1 criteria in the 0.5 0.9 ng ml−1 range.
Specimen At the Point A At the Point B Deflection Support rotation Comparison to criteria Support rotation Comparison to criteria BC 17.18° NG 37.56° NG Failed BC-F 2.81° NG 17.24° NG Failed BC-D 0.99° OK 9.91° NG Controlled BC-F-S 1.15° OK 2.76° NG Controlled BC-A 0.55° OK 2.15° NG Controlled Table 8 Maximum stress and strain in reinforcements.
