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Our findings showed that treatment with RANKL (100 ng) at day 3 significantly increased caspase-9 activation in WT osteoclast precursors when compare to TIEG1−/− precursors (Figure S1A) suggesting that TIEG1 mediates RANKL-induced caspase-9 cleavage in osteoclasts.
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The defined groups were as follows: Group 1a patients with TTL<50 ng ml−1 at day 15 and no relevant toxicity (n=5; 17%), Group 1b patients with TTL<50 ng ml−1 at day 15 with relevant toxicity (n=10; 34%), Group 2a patients with TTL>50 ng ml−1 at day 15 and no relevant toxicity (n=8; 28%), Group 2b patients with TTL>50 ng ml−1 at day 15 with relevant toxicity (n=6; 21%).
As shown in Figure 3, the released amounts of BMP-2 from BMP-2/PCL and BMP-2/AAc-PCL were 57.07 ± 0.02 ng and 39.12 ± 0.03 ng at 1 day, respectively.
Transduction of MSCs with adenoviral vectors encoding BMP-2 or BMP-4 using viral doses sufficient to generate 30 to 60 ngs transgene product at day 3 induced a significant chondrogenic response in the respective aggregate cultures compared with the controls, which were not chondrogenic.
The 14 patients that had undergone dose escalation after day-15 PK measurement (median TTL 42.0 ng ml−1 (IQR 36.3 47.3)) reached median TTL of 51.3 ng ml−1 (IQR 44.7 58.7) at day 29.
injected with sublethal doses of Stx2, 0.25 and 0.5 ng Stx2/g of body weight (bwt), at day 8 of gestation (early postimplantation period of gestation).
Total of 166 ng small RNAs from 3 placentas at day 50 derived from every group of pregnancy (IVP, NT and AI) and donor cells (in triplicate) were synthesized into first strand cDNAs using RT miRNA first strand kit (SABiosciences).
In other mice, 100 ng of LPS was administered i.p. at day 3 after infection.
Values increased to 2 ng/ml at day 2 and remained high up to 7 days after injection (Fig. 1a).
Subsequent release resulted in a peak level of 20 ng/mL on day 8 after which orntide levels decreased to approximately 3 ng/mL and remained there for at least 60 days before decreasing gradually to below 1 ng/mL at day 109.
*P < 0.05 compared to the date at day 0. In order to further investigate in vivo toxicity of MTX-CS NGs, a histological analysis of rats' spleen was performed to determine whether MTX-CS NGs caused tissue damage (Fig. 7).
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