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The typical yield of SH-SY5Y was 700 ng and of SK-N-MC 5 μg of total RNA per well in a 6-well plate. 1 μg muscle total RNA or 200 ng neuroblastoma cell total RNA, in reaction volumes of 40 μl was reverse transcribed using RT-PCR kit (Applied Biosystems) according to the manufacturer's protocol.
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The combined data, from the overexpression of Ng and that of Ng knockdown, strongly suggest that Ng is required and rate-limiting for LTP induction.
These differences in the level of Gag-specific total IgG elicited by booster doses of 646 ng and 64 ng of p17/p24 protein were significant (p < 0.05).
Photos courtesy of Pak Tee Ng and National Institute of Education, Nanyang Technological University, Republic of Singapore.
Real time quantitative PCR assay allowed the detection of as few as 0.01 ng and 0.05 ng of ovine and bovine genomic DNA, respectively.
Larvae were injected with approx. 0.1 ng and 1 ng of alpha-amanitin respectively, and these concentrations of alpha-amanitin caused no mortality or toxic effects.
The detection limits of the three pairs of T. lewisi-specific primers were 50 ng, 1 ng and 10 ng of total DNA, respectively.
As positive controls 10 ng, 100 ng, and 1000 ng of the G. graminis LOX were applied.
50 ng and 20 ng of indicated proteins were spotted on nitrocellulose membranes.
Where indicated, 100 ng and 200 ng of IKKβ KM vector has been used.
Standard curve concentrations were set at 100 ng, 50 ng, 25 ng, 12 ng, 6 ng and 3 ng of total RNA.
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