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The purpose of EPJ TI is to catalyze the development and adoption of next-generation techniques and technologies.
This technique has evolved from pioneering studies utilizing microarray hybridization techniques to score presence/absence of marker regions adjacent to restriction sites in the genome [23], to exploit next-generation techniques to directly sequence these regions [6].
SRAP markers may also prove valuable when paired with the large-scale capability of next-generation techniques.
For more in-depth information on each of these technologies, see Loman et al. (2012a, b).> There are a number of next-generation techniques available with associated advantages and disadvantages to each technique depending on the desired application (Table 1).
High-throughput sequencing technologies now play a significant role in microbial ecology studies [ 10] and next-generation techniques such as 454 pyrosequencing facilitate the detection of unculturable microbial communities [ 11, 12].
In this method, forward and reverse primers are first developed for each putative microsatellite fragment (e.g., using next-generation techniques or following traditional methods such as Zane et al., 2002).
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Some innovative next-generation technique must be applied to solve the problem completely.
Fluid modelling is a next-generation technique for analysing massive performance models.
So far, however, there has only been analysis of dominant microbes, through relatively insensitive techniques such as qPCR, without assessing the impact on subdominant populations as allowed by next generation techniques such as t-RFLP.
Results were compared against a curated fungal sequence database maintained by Research and Testing Laboratories, Inc. Fungal ITS sequences were subjected to phylogenetic analysis to explore similarity between taxa amplified with varied primers and sequenced by conventional and next generation techniques.
There has also been a surge in other next generation techniques such as RNA-seq, incorporating experimental methods gives a better indication of a protein's role and whether it is functional.
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