Results: Using a human whole exome enrichment technique, chimpanzee and rhesus macaque samples were captured alongside a human sample and sequenced using standard next-generation methodologies.
Next-generation methodologies that build upon these strategies will bundle together the key components of these methodological advances to provide a powerful tool for quantitative descriptions of the phenotypes of this genetic model organism.
This is particularly important for the rapidly expanding set of genomes, like that of the turkey, that are shotgun sequenced solely by "next-generation" methodologies that tend to give shorter sequence contig and scaffold lengths.
iAs a result of technological advances (especially the introduction of next-generation sequencing methodology), the single-organism approach has recently succeeded in shifting its emphasis from research on traditional model species to ecologically-interesting species, e.g. the water flea Daphnia Pulex.
We adapted the next-generation pyrosequencing methodology (454/Roche) for phage DNA; for proof-of-principle, we used tissues obtained after intravenous administration of a phage library to end-of-life patients [18] [20].
Next-generation sequencing methodology has been applied to sequence RNA from 20 tissue samples.
All these factors would render topology-based approaches to be a next-generation benchmark methodology for pathway-based analysis of both microarrays and GWAS data.
This endeavor has been followed by sequencing efforts using next-generation sequencing methodology, which provided a wealth of information, particularly for species in the Saccharomyces complex (Dujon et al., 2004; Cuomo and Birren 2010).
Collectively, the completion of the Human Genome Project and subsequent development of high-throughput next-generation sequencing methodologies have revolutionized genomic research.
Extensive genetic analyses, recently using high-throughput molecular techniques and next-generation sequencing methodologies, and the development of sophisticated genetically engineered mouse models closely recapitulating human disease, have improved our understanding of the genetic basis of pancreatic neoplasms.
