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Results: Using a human whole exome enrichment technique, chimpanzee and rhesus macaque samples were captured alongside a human sample and sequenced using standard next-generation methodologies.
Next-generation methodologies that build upon these strategies will bundle together the key components of these methodological advances to provide a powerful tool for quantitative descriptions of the phenotypes of this genetic model organism.
This is particularly important for the rapidly expanding set of genomes, like that of the turkey, that are shotgun sequenced solely by "next-generation" methodologies that tend to give shorter sequence contig and scaffold lengths.
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In conclusion, a "next-generation" methodology able to assess embryo competence, improving IVF success rates and reducing the likelihood of multiple pregnancies, is urgently required.
Collectively, the completion of the Human Genome Project and subsequent development of high-throughput next-generation sequencing methodologies have revolutionized genomic research.
Extensive genetic analyses, recently using high-throughput molecular techniques and next-generation sequencing methodologies, and the development of sophisticated genetically engineered mouse models closely recapitulating human disease, have improved our understanding of the genetic basis of pancreatic neoplasms.
Finally, quantitative-PCR plus next-generation sequencing methodologies have not as yet been systematically compared to TU-counting plus Sanger sequencing in terms of speed, cost, and, most importantly, accuracy.
Next-generation sequencing methodologies, along with the relevant amplification protocols are rapidly evolving.
With the rapid development of DNA microarray technology and next-generation sequencing methodologies, analysis of the transcriptome is now commonplace.
Advances in next-generation sequencing methodologies have significantly reduced the time and cost constraints of determining genome-wide expression levels of various organisms, including bacteria.
With the next-generation sequencing methodologies, DNA sequences can be retrieved even from samples (for example human remains) for which the technical pitfalls of classical methodologies required stringent criteria to guaranty the reliability of the results.
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