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Genome editing via clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) constitutes a next-generation method for programmable and high-throughput functional genomics.
In using the next-generation method, it is necessary to identify a suitable infected population in the model to choose as an initial infected population.
The population V comes only from one population (the I population), and therefore we choose it as the initial infected population for the next-generation method and order the variables as ([V,T,L,I,W]^{T}).
They are: (1) Lyapunov's first method of checking the eigenvalues of the Jacobian of the linearized system at an equilibrium point (see, e.g., [19]), (2) the next-generation method of van den Driessche and Watmough [20], or (3) by finding the condition for the existence of the chronic equilibrium as in equation (9).
Compared with this alternative next-generation method, solexa has the advantages of lower cost and generation of larger amounts of data [ 17].
The linear stability of E v0 is governed by the basic reproductive ratio R v. Using the next-generation method [ 31], we have and The basic reproductive ratio is the spectral radius ρ(FV 1) which is (5) Lemma 1 The disease-free equilibrium E v0 is locally asymptotically stable if R v < 1, and unstable if R v > 1. Proof.
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Doris Sung is an award-winning artist, architect, and researcher whose work explores next-generation methods of design and fabrication.
There are multiple sequencing methods that are utilized in next-generation methods.
Once these targets are captured they are sequenced using next-generation methods [ 25].
But what, if anything, do these next-generation methods add to traditional single-gene and gene-set approaches?
These regions can be amplified by PCR and sequenced by next-generation methods using a multidimensional pooling strategy.
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