Rapid computational methods are needed to comprehensively detect and characterize specific classes of structural variation using next-gen sequencing technology.
I will be exploring lot of avenues for handling, analysis and interpretation of high-throughput next-gen sequencing data.
Established methods for measuring genome-wide chromatin organization use next-gen sequencing as a proxy for spatial measurement.
For example, next-gen sequencing was supposed to take six decades by Moore's law, which is a pretty aggressively optimistic law.
Around 1999 I felt that there was some possibility we'd be sequencing human genomes with next-gen sequencing — plural, not just singular.
Our lab takes a multi-disciplinary approach to exploring these questions, and employs a wide range of biochemical, bioinformatics, and next-gen sequencing strategies.
"The combination of high-throughput synthesis and next-gen sequencing allows us to answer big, complicated questions that were previously impossible to tease apart," Church said.
I can't say that I had conceived of every nuance of next-gen sequencing in that thesis, but there were some pretty significant components.
In next-gen sequencing, scientists grind up tissue, extract its DNA, break the DNA into pieces, then dilute those pieces enough so that each piece of DNA sticks to a separate spot on a glass slide.
To this end, we use an interdisciplinary approach combining molecular techniques (ranging from next-gen sequencing and transcriptomics to cell-based pharmacological assays and in vivo viral vectors), population-genetic tests, classical genetic crosses, lab-based behavioral assays and field-based experiments.
At SGTC, he developed the first semiconductor sequencing platform and first base-calling algorithm for next-gen sequencing.
