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Contig MDC015568.236 contained sequences most similar to the R5 next-gen sequences, and was therefore chosen for further analysis.
Geneious bioinformatic software (Biomatters, San Francisco, CA, USA) was used to construct a local alignment of next-gen sequences to the Malus × domestica contig containing the complete predicted target gene sequence (MDP).
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Rapid computational methods are needed to comprehensively detect and characterize specific classes of structural variation using next-gen sequencing technology.
Established methods for measuring genome-wide chromatin organization use next-gen sequencing as a proxy for spatial measurement.
I will be exploring lot of avenues for handling, analysis and interpretation of high-throughput next-gen sequencing data.
Around 1999 I felt that there was some possibility we'd be sequencing human genomes with next-gen sequencing — plural, not just singular.
Part of his work is related to the practical aspects of processing next-gen sequence data, including read mapping and variant calling.
For example, next-gen sequencing was supposed to take six decades by Moore's law, which is a pretty aggressively optimistic law.
I can't say that I had conceived of every nuance of next-gen sequencing in that thesis, but there were some pretty significant components.
Our lab takes a multi-disciplinary approach to exploring these questions, and employs a wide range of biochemical, bioinformatics, and next-gen sequencing strategies.
"The combination of high-throughput synthesis and next-gen sequencing allows us to answer big, complicated questions that were previously impossible to tease apart," Church said.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com