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The Bus 2 voltage calculated by (6) will be next used for PLL calculation as shown in Fig. 5.
MCF-7 cells were next used for CYP1A-related EROD activity assay and methylene blue assay.
cDNA corresponding to approximately 5 ng of initial RNA was next used for each quadruplicate quantitative PCR reaction.
Lysates from cells transfected with V5-tagged mortalin mutants were next used for immunoprecipitation of Bcl-2 and Bcl-xL.
These transcripts were used to create a final database containing 13,847 putative gene models next used for the generation of a training set for ab initio predictors and for the correction of predicted gene models.
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We next used GEE for logistic regression to develop the probability function for significant OAG progression.
We next used MEFs deficient for Bim and Puma.
We next used the antibodies for immunoblot analysis using COS7 cell lysates transfected with TMEPAI/V5 or C18ORF1/V5 expression plasmids.
We next used the estimated CDS for each unigene to calculate the codon position for each SNP.
These N-functionalities have been next used as anchoring points for the grafting of Au nanoparticles.
We next used BRET to look for interactions between β2ARs during internalization.
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