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Substrate 8b, where formation of the carbanion adjacent to the ring nitrogen of the oxazolidinone is blocked by substitution, was next treated with potassium ethoxide (0.1 eq), in the presence of 18-crown-6 ether, at room temperature (5 days), and gave rise to 9b in 55% yield (cf. Scheme 8).
The sections were next treated with avidin biotin complex, followed by a DAB substrate stain according to the manufacturer's directions (Vectastain Elite ABC kit; Vector, Burlingame, CA).
To extend this observation to biological fluids, C. neoformans yeasts (1×105) were next treated with undiluted bronchoalveolar lavage fluid (BAL).
The sections were next treated with FITC-conjugated anti-pimonidazole mAb (Natural Pharmacia International Inc).
In this case, SUDHL-2 cells pretreated with INK128 were next treated with sodium arsenite.
SaOS2 cells expressing ΔNp63 α, WWOX or both together were next treated with cisplatin for 48 h.
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We next treated embryos with elongating notochord cells with blebbistatin for 3 hr.
To determine if menin half-life is extended following DNA damage, we next treated cells with γ-IR and added the protein synthesis inhibitor cycloheximide (CHX).
We next treated mice with 25 mg/kg STLC and investigated mitotic arrest after 3 hr.
We next treated cells with bromodeoxyuridine (BrdU) and measured its incorporation.
Because Gi/o is known to activate PLC (Exton 1996), we next treated cells with PLC-selective inhibitor, U73122, which led to a marked DEP MMP-1 response.
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