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Next, to understand the molecular mechanisms by which TRIM28 increases TRIM24 protein levels, we first performed qRT-PCR analysis and found that TRIM28 knockdown did not decrease TRIM24 mRNA, precluding transcriptional regulation (Fig. 1e).
Next, to understand changes of human well-being yielded from ecosystem services and GDP in biodiversity hotspot and non-hotspot countries, respectively, we compared roundwood production, hydroelectricity generation, tourism investment and GDP in the two categories of country.
Next, to understand the complex dynamic interplay of the various intracellular signaling molecules in the regulation of NF-κB, JNK and p38 in poly (I∶C) stimulation, we developed a computational model of the TLR3 signaling (see Materials and Methods).
Next, to understand how EZH2 regulates neuron differentiation of hMSCs, we wanted to identify the target genes of EZH2.
Next, to understand the significance of the DAVID enrichment score, 1000 genes were randomly selected and used as an input for DAVID.
Next, to understand if Pam3CSK4 alone or its combination with Velcade influenced the enzymatic activity of caspase-3, we assayed its activity using a specific peptide substrate.
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What's the big next thing to understand?
Cavanagh was too busy trying to be the next Nick Hornby to understand that.
"Well, I think it's really important for the next president to understand the economy".
That was always the thing with me - finding the next thing to understand.
We next sought to understand how these knockdowns were activating a 2C gene expression program.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com