On the other hand, in recent years, the role of rare genetic variants has received more attention due to the advent of next generation sequencing (NGS) technology.
In recent years, the number of causative genes associated with non-5q SMA has expanded rapidly due to the advent of next generation sequencing technologies.
Recently, emphasis on EST sequencing has waned due to the advent of next generation sequencing techniques that can quickly dissect a transcriptome.
Prior to the advent of next-generation sequencing, clonality was usually determined by cytogenetic analysis.
Prior to the advent of next-generation technologies in nucleotide sequencing and mass spectrometry, molecular studies of entire microbial consortia (rather than selected organisms of interest) were not technically feasible.
Previous to the advent of next-generation sequencing (NGS), microparasites that were difficult to culture could exist for decades with no identified agent.
The latter group of disorders was described in detail prior to the advent of next-generation sequencing (Steinlin, 1998; Steinlin et al., 1998) and include patients with or without cerebellar hypoplasia (as defined in Poretti et al., 2008).
Moreover, as the amount of specificity data is increasing swiftly, also due to the advent of next-generation sequencing and its applications to phage display [ 69], our method will prove even more valuable to make optimal use of this kind of data.
At the next UK election the advent of YouTube et al is certain to mean that parties will experiment with online political adverts.
Next steps Since the advent of free primary education in 2003, throngs of children have gone to school all over the country, some for the first time.
