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Comprehensive candidate gene screens of natural mosquito populations represent a logical and promising next step to identify a broader spectrum of genetic variants important in insecticide resistance and could also pave the way for subsequent genomewide association (GWA) studies.
Hence, follow-up regionally-focused sequencing of the most compelling of these regions is a cost-effective and logical next step to identify the critical underlying risk variant at these loci.
I agree with the authors that correlating genomes with phenotypic variation may be the next step to identify potential candidate genes that may have a role in complicated behaviours such as migration.
19, 20 Since M-ARMS can detect only a given set of mutations specific to the primers employed, direct DNA sequencing is the next step to identify various point mutations and small rearrangements in the beta-globin gene.
In future studies, we plan to examine the candidate genes for suggestive polymorphisms, and perform expression assays for these and other candidate genes under control and challenge conditions as the next step to identify the actual genes involved in resistance.
The next step to identify the 'system' of genes that work together to influence cell-specific determination and differentiation will require the use of large gene expression datasets and potentially additional dataset types such as protein-protein interaction datasets, ChIP-chip datasets, datasets from animals with specific mutations, etc.
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The in-depth characterization of the transcriptomes using RNA-seq and whole genome sequence analysis of N33, currently underway, is the next step to identifying new cold adapted genes in strain N33.
Optionally, we next perform the FBS step to identify 'Secondary Alignments' that have a high length overlap and score agreement with a primary alignment (Fig. 1D).
The next step is to identify your main archetype.
When genes of interest have been isolated by the bead system, the next step is to identify them.
The next step is to identify versions of the genes that lead the tomato plant to produce more of them.
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