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We expanded our sequencing analysis and next sequenced SETD2 in 123 additional gliomas of various ages and grades (Table 1; Table S1).
We next sequenced the exons covering codons 12, 13, and 61 in HRAS, KRAS, and NRAS and detected a total of six mutations in HRAS, four in KRAS, and none in NRAS (n = 218).
Thus, PICRUSt predictions depend on the topology of the tree and the distance to the next sequenced organism.
The figures shown in the graph are therefore the number of new clusters generated by the 400 next sequenced clones.
For somatically mutated genes, we next sequenced the remaining coding exons from the 40 discovery screen tumors and sequenced all coding exons from another 72 ECs (10 clear cell, 21 serous, 41 endometrioid).
DNA was next sequenced using Sequonom MassArray mass spectrometry technology (Sequonom, Inc., San Diego, CA, U.S.A) to assess for mutations in particular point mutations including but not limited to KRAS, NRAS, BRAF, PIK3CA, and CTNNB1 (see Additional file 1: Table S1 for full listing of genes sequenced).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com