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The RT-PCR product was used for the next round of selection as described above.
Such assembled DNA pool was then used for the next round of selection.
The DNA pool collected after each round of selection was amplified by PCR, and the product was used to prepare ssDNA for the next round of selection.
The resulted DNA was purified, and served as template DNA for next round of selection or cloned using a TOPO TA cloning kit (Invitrogen) followed by sequencing with an ABI PRISM 3100 genetic analyzer (Applied Biosystems).
The precipitant was diluted by adding 50 µL sterile ddH2O, boiled for 5 min, snap-cooled on ice for 3 min, extracted by phenol/chloroform (25∶24), and the supernatant was used as the template for PCR to obtain the ssDNA pool for the next round of selection.
A gene or interaction with high fitness will survive in the next round of selection.
Similar(38)
A few, judiciously chosen appointments in the next round of selections to the UK's supreme court could change public perceptions rapidly.
He knew that captains normally required a certain amount of seagoing command experience to be considered, but because his tour of duty on Indianapolis had been cut short in order to take up the post in Rome, he did not have enough months, and it was unlikely that a billet as captain of a battleship or cruiser would come up in sufficient time before the next round of selections.
Those sequences that bound to the cells were separated, amplified using polymerase chain reaction (PCR), purified using gel electrophoresis, and served as the input DNA pool for the next round of SELEX selection.
Then, if mean annual temperature was the most influential and significant temperature-related variable, all other temperature-related variables would be excluded in the next round of model selection.
The treated mouse containing the highest parasitaemia on day 7 p.i., was then exsanguinated by section of the brachial artery under general anesthesia and served as donor for the next round of drug selection.
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CEO of Professional Science Editing for Scientists @ prosciediting.com