Sentence examples for next round of error-prone from inspiring English sources

This plasmid DNA was used as the template for the next round of error-prone PCR.

The best recombinant from each round was then used as the parent for the next round of error-prone PCR.

The variant with the largest improvement in initial rate was then used as the template for the next round of error-prone PCR or several variants were subjected to DNA shuffling.

Plasmids from this clone were isolated, sequenced, and subjected to a new round of error-prone PCR and selection.

After a single round of error-prone PCR, the variant Pro15Ser exhibiting modified substrate specificity was generated.

Distance measurements of the A70T mutation, which arose in the first round of error-prone PCR (Dieselzyme 2), shows that the hydroxyl from the threonine forms a new H-bond interaction with T68 and the backbone carbonyl of K72.

Similarly, the R33T mutation that arose after the final round of error-prone PCR (see Dieselzyme 4) forms new H-bond interactions between the threonine hydroxyl and the backbone carbonyls from A29 and D30.

Fan et al. generated a library of variants of a fungal chitinase after three rounds of error-prone PCR and DNA shuffling.

We first performed three rounds of error-prone polymerase chain reaction and identified mutations at six sites (F110, A255, E349, R228, T249, and I352) that influence catalytic efficiency.

After two rounds of error-prone PCR and backcross with parental DNA, three mutants were identified for improved activity toward pyrene and for the first time a new activity toward chrysene in comparison to the wild type enzyme.

For each condition, six independent replicate lines were subjected to four rounds of error-prone PCR followed by the selection in a range of concentrations of one or both antibiotics.