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ChIP assays were next performed on >isolated mouse colonocytes.
Immunohistochemical analysis was next performed on the xenograft sections (Fig. 5B).
Final confirmation came early Wednesday morning from a polymerase chain reaction test performed on samples sent to C.D.C. in Atlanta.
We next performed immunoblot analysis on identical samples to determine whether the difference in the expression of HIF-1α, HIF-2α and DDX3 mRNA in these cell lines influences their protein expression.
To test whether CpG methylation in TCF4 exon 1 is abnormally increased in primary tumors compared with the corresponding normal tissue, we next performed MSP analysis on clinical samples.
We next performed in vitro growth studies to assess the effect of curcumin on HIMEC growth.
We next performed PCR on the DNA extracts with primers specific to the mitochondrial coding regions.
To validate this finding we next performed northern blotting on RNA derived from hNPC and hNPC-N samples on four independent donors.
To determine whether combinations of cytokines and autoantibodies might provide superior differentiation of pretreatment samples derived from etanercept responders and nonresponders, we next performed SAM analysis on integrated antigen array and cytokine datasets obtained for the 29 ABCoN samples.
We next performed generic EMT scoring on multiple clinical samples and cell lines (Fig 3, Supplementary Fig S6, Supplementary Table S4A D).
We next performed rarefaction analysis on the resulting spacer groups to get an estimate of the spacer richness in each subject or group of subjects and to determine whether the majority of the spacer sequences had been sampled.
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