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We next obtained up-to-date (23JUL08) GO annotations for, and then modeled based on GO biological process (GOBP), these differentially expressed proteins.
We next obtained log2ratios between tumour and normal samples and used Oncodrive to identify genes that are significantly upregulated in this set of tumours (Figure 2D).
An eight-time tag-repeated sequence was next obtained by insertion of the XhoI/EcoRI small fragment into the SalI/EcoRI large fragment.
A fraction enriched in epithelial cell aggregates (organoids) was next obtained by centrifugation of the dissociated tissue at 80 g for 30 seconds and then washed in fresh DMEM/F12 medium at least 3 times.
We next obtained a ranked list of candidate in vivo direct COUP-TFI targets by integrating the microarray and bioinformatics analyses according to the degree of binding site evolutionary conservation and microarray statistical significance.
We next obtained transcriptomes from the four isolates.
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We next obtain the following theorem.
Let us next obtain an explicit expression of R t i for use in (14) and (15).
Substituting the parameters into the mathematical solutions [Equations (4), (10), and (11)], which are shown in Method, we next obtain structural properties from this model.
Next we obtain the following result as regards (E_{2}(S^,I^)).
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