Sentence examples for next incubated with from inspiring English sources

Membrane was next incubated with antibody solutions (anti-Dig-AP antibody in Blocking solutions) for 30 min and was washed twice for 15 min by wash solutions.

Cells were next incubated with primary antibodies diluted 1∶1000 in PBTG (PBS, 1% Goat serum, 0.2% TritonX-100) for 2 hours at room temperature.

After having been rinsed with PBS, they were next incubated with horseradish peroxidase (HRP -conjugated anti-goat Igs (CHRP -conjugatedtional Inc).

Slides were next incubated with anti-α-synuclein antibody 4D6 at dilution 1∶100 for 1 h at room temperature, followed by washing with PBS.

Cells were next incubated with perhexiline, niclosamide, amiodarone, rottlerin or rapamycin in the absence or presence of bafilomycin A1 and EGFP-LC3 processing and degradation was monitored by western blotting.

Stained embryos were washed with 1XPBS-T (0.1% Tween sixx times over a three hour period (30 minutes each) and were next incubated with the appropriate secondary antibodies for 3 hours at room temperature.

We next incubated macrophages with exogenous Hsp70 and then measured NF-κB activation in these cells after 2 h using anti-p65 antibody followed by labeling with fluorescently tagged secondary antibody.

To investigate more directly the role of MAO, we next incubated myoblasts with tyramine, a substrate for both MAO-A and MAO-B.

We next incubated heme with purified human p53 protein (see Figure 3D for purity of the protein) to reconstitute the heme-p53 complex in vitro, using gel filtration in size-exclusion chromatography (SEC) to remove unbound heme.

To specifically assess the activity of N-terminal annexin A1 derivatives released from apoptotic polymorphonuclear leukocytes (PMNs), we next incubated monocytes with either the conditioned medium of apoptotic neutrophils (as before, Fig 1) or the annexin A1 peptidomimetic Ac2-26, whicorrespondsnds to the N-terminal domain of annexin A1.

In order to determine the alterations in F-actin cytoskeletal organization associated with the above changes in cell shape, we next incubated the cells with tetramethylrhodamine 5-isothiocyanate (TRITC -labelled phalloidin in order TRITC -labelledn in the different treatments.