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In the knock in strain, a 1-kb band corresponding to the chloramphenicol cassette was amplified whereas in the WT strain a 1.2-kb band corresponding to the next gene of argT gene (STM2356) was observed.
DXR (MELO3C026292), the next gene of the pathway, was also up-regulated during the later fruit developmental stages (Additional file 1: Figure S5).
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Sequence analysis of this 1,721 bp DNA fragment indicated it contains not only the remaining sequence 5' of BjMTP1 but also partial sequence of the next gene upstream of BjMTP1 which showed 88% similarity to the A. thaliana gene locus At2g46790 (unknown protein).
In Fugu rubripes, the next gene downstream of NUP62 is a homolog of human integrin alpha 6, and the two genes are oriented tail to tail.
The next gene downstream of dmr6-like, MDP0000927098 (ATL5K-like), and one other downstream gene, MDP0000163720 (ACC1-like), are also induced in shoot apical meristems of columnar P28.
The next gene centromeric of this, Luzp2, showed ~0.8-fold decreased mRNA levels in TgPWS brain at P1 and P4 and in TgAS brain at P4 (P < 0.05) (Fig. 3D).
By tissue culture, in vivo transgenics and analysis of chromatin structure, we have found that this region contains potentiator cis-regulatory elements that interact with the promoters of Pitx2c and, unexpectedly, Enpep, the next gene located downstream of Pitx2.
Termination of each gene is required to allow transcription of the next gene downstream, and the propensity of the polymerase to dissociate from its template results in transcriptional attenuation at each gene junction (Fearns and Collins, 1999).
Between the start codon of the PB103464.00.0 gene and the next gene upstream, a region of 989bp is present.
Each of the first three genes inhibits the activity of the next gene down the line.
The 1325 bp 3'-UTR region includes the minimal 3'-UTR region plus partial sequence of intron region next to the 3'-UTR of the next gene At4G05400.
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