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Next, forward and reverse SNP flanking primers were used for high resolution melting (HRM) analysis by real-time PCR.
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Subsequently, the sliding window shifted based on the previous reverse primer, and the next forward primer was designed from the 20-bp upstream region of the previous reverse primer (Fig. 5).
After this backwards step, the next forward step proceeds.
When I was a child I wanted to be... England's next centre forward and score a goal at Wembley in the Cup Final.
"He gave the encouragement to so many people of color to make that next step forward and not to be afraid.
It's a demand that comes precisely at the point where the learner is capable of taking the next steps forward – and helping the learner meet that demand, rather than ignore it.
And he warned the audience in Munich that "you need to get a national consensus around the preconditions of the next step forward," and that, in the remarks that so angered Mr. Obama, Mr. Mubarak "must stay in office in order to steer those changes through".
It takes the next step forward and continues that process.
By my junior year, I began thinking about the next step forward and decided to pitch to local publications as a freelancer.
Bring the next section forward and repeat.
This means that imbuing individual atoms with a 0 or 1 is the next major step forward and the next major barrier in storing data digitally, both increasing capacity by orders of magnitude and presenting a brand new challenge to engineers and physicists.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com