Sentence examples for next blocking from inspiring English sources

Next, blocking was performed with blocking solution for 30 min at room temperature (Roche) and rabbit immunoglobin fraction (Dako).

Next, blocking of MCP-1 with neutralizing antibodies for 30 min before infection also down-regulated CCR2 compared to no antibody controls using fluorescent microscopy (Figure 3B).

Next, blocking solution of 10% horse serum in 1% PBS-Triton-X-100 was applied for 30 min at room temperature.

These competitor antibody mixtures were incubated at room temperature until the next blocking step was done.

Next, blocking for unspecific peroxidase activity was done by 3% H202 treatment for 5 min. Primary antibody detection was performed, in accordance with the manufacturer's instructions, with the MACH3 mouse probe (Biocare Medical) for 20 min, followed by MACH3 HRP polymer (Biocare Medical) for 20 min, and the signal was developed with diamino-benzidine DAB+ (Dako) for 5 minutes.

Next, Blocking Solution (300 μL) (Protein-Free (TBS) and Blocking Buffer from Thermo Fisher Scientific) were added to each well, and the plates were incubated at room temperature for 1 h on a shaker to block nonspecific binding.

To further evaluate our result, we next blocked the ROS production in the CpG treated DCs and examined Salmonella proliferation.

We next blocked Rac1, an activator of the NOX complex.

Brains were next blocked into 5-mm sections containing somatosensory cortex (Paxinos and Watson 2007).

The next block was also destroyed.

The next block up houses the Mayflower Hotel.