Exact(11)
To further evaluate our result, we next blocked the ROS production in the CpG treated DCs and examined Salmonella proliferation.
We next blocked Rac1, an activator of the NOX complex.
Brains were next blocked into 5-mm sections containing somatosensory cortex (Paxinos and Watson 2007).
Tissues were next blocked with 10% normal donkey serum (NDS) at room temperature for 1 h followed by incubation with primary antibodies at 4°C for 16 h.
Cells were permeabilised, next blocked with rat serum and stained with anti-FOXP3 Alexa Fluor 488 (BioLegend) and relevant isotype rat controls.
We next blocked the different MAPK signaling pathways with the p38-specific inhibitor SB203580, the ERK1/2-specific inhibitor PD98059, and the JNK1/2-specific inhibitor SP600125 in the culture medium.
Similar(49)
These competitor antibody mixtures were incubated at room temperature until the next blocking step was done.
The next block was also destroyed.
The next block up houses the Mayflower Hotel.
The officer pointed to a colleague in the next block.
I'll post their response in the next block.
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