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The sensitivity to 5FU was assessed in selected plants in order to establish the RNAi silencing efficiency of the DUT1 gene in these new transformants (Figure 8B).
In an attempt to isolate a further improved itaconic acid producing strains, new transformants were generated.
Currently, we have produced more than 22,000 moss transformants; the current production capacity is about 3,000 new transformants per month.
X-chromosomal insertions that were homozygous lethal or sterile could be obtained only if the following is considered: Because only new transformants that segregated away from the helper element were selected, hybrid females had to be used to set up P2 crosses in order to avoid bias against new X-linked insertions.
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Second, genomic DNA from a Shi470 ΔrdxA-cat transformant yielded ∼10,000 new Camr transformants of its isogenic wild type parent (vs. only ∼60 obtained using DNA from strain Shi18 ΔrdxA-cat).
Shake flask cultures are commonly used for screening of new A. niger transformants [ 10, 17].
DH5α chemically-competent E. coli cell (New England Biolabs) transformants were plated overnight at 37 °C on 100 μg/ml ampicillin (AMP) agar plates.
In order to improve transformation efficiency and to achieve higher GFP expression levels as well as stable transformants, a new series of cloning vectors for ATM transformation was constructed.
We developed a host vector system for the wastewater treatment yeast, H. fabianii J 640, and we created new wastewater treatment yeast transformants (HF-XYN and HF-AAMY).
Eight transformants carrying new codon 3 alleles but no other mutations elsewhere in fur were identified by DNA sequencing, and were tested quantitatively for levels of Mtz resistance.
Both genes are dominant because they add new, selectable features to transformants, and therefore do not require strains with particular auxotrophic defects as recipients.
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