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We could not detect the potentially aberrantly spliced product from the new splice donor site predicted for the c.436C>T mutation.
If the new splice variant is advantageous, selection might operate to optimize the new splice sites and consequently increase the proportion of the alternative splice variant (Schmitz and Brosius, 2011).
We found that this method defined many new splice junctions.
This suggests that the intron 4 containing N-terminal truncation is necessary for the inhibitory action of the new splice variants.
New splice sites have been used in 58% of the CGs; however, in the majority of these cases (85%) splicing occurred at canonical sites (GT-AG).
In this report, we have shown that CXCL12γ, a new splice variant of CXCL12, displays an unusually high affinity for GAGs and investigated the structural determinants involved.
Obviously, identifying new splice events is difficult using this approach.
Tissues with large numbers of new splice forms also had a larger fraction of candidate new splice forms.
How the new splice sites are recognized also appears to be tissue specific.
In this study, we found a new splice variant designated CD46 transcript variant (CD46-TV).
Thus, this method cannot be used to identify a new splice site.
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