Sentence examples for new ligation from inspiring English sources

Recently, there has been significant progress in the improvement of classic ligation procedures as well as in the establishment of new ligation methodologies.

Alongside developments in enzyme technology, new ligation strategies have been developed.

Here, we propose that a new, ligation based probe assay can bridge the gap between highly specific target recognition and high-throughput, multiplex pathogen quantification.

However, the new ligation independent cloning still requires purification of the digested vector and PCR-amplified insert, and the purchase of purification and cloning kits.

When this ratio was determined, new ligation reactions were set up (four reactions, 500 μl each) and used for the transformation in yeast.

The proposed modelling methodology can be a useful tool to obtain the structure-activation barrier relationships of bio molecules and thus propose new ligations in click chemistry.

With our new ligation-based probe production, we introduce cost-efficient probe production with possibilities for innovation and potential increase in efficiency.

If necessary, new ligations were performed to give a final library of approximately 106 clones: the titer was estimated on Chloramphenicol LB plates.

This new bioorthogonal ligation could facilitate important advances in the understanding of protein glycosylation.

With the new auxiliaries, peptide ligation could be conducted at non-Cys and non-Gly sites.

The pools were then carried through the standard Illumina library preparation process using Adaptive Focused Acoustics for shearing (Covaris), end-repair, A-tailing and ligation (New England Biolabs).