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We generated 4613 new ESTs from the adult zebrafish testis and ovary and 11010 sequences from the differentiating gonad [3, 4 and 5 weeks post-fertilization (wpf)], which has never been sampled before by others (refer to Table S1 for details).
In the second version of BmiGI, the total number of new ESTs sequenced was 22,095.
In addition, we obtained 100,000 new ESTs from five more species.
Using the new 454 pyro-sequencing technology, we have provided the so far largest collection of new ESTs for cichlid species.
Indeed, we obtained about 100,000 new ESTs in total for five different aphid species, to evaluate gene expression and sequences in these different species.
TCs group EST sequences that can be aligned and clustered in distinct groups, and these are periodically updated as new ESTs from GenBank become available.
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This paper reports the development of a set of new EST-based SSR assays.
These new EST-SSR markers would provide sufficient polymorphism for population genetic studies and genome mapping of the common carp and its closely related fishes.
These new EST-SSRs markers will be useful for genetic diversity analysis, genetic mapping construction and gene discovery in Soja subgenus.
Our study is aimed to generate new EST-SSR markers and to evaluate their potential for cross-species utilization in phylogeny study of genus Dendrobium.
The 54 new EST-SSR primer pairs comprising 540 alleles were used to investigate cross-species transferability among 26 accessions, which represented 8 species (three exotic cultivated species and five natural wild species from China) from 6 sections of Vaccinium.
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