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We have now developed a new complemented Kin line expressing HASPB and SHERP proteins at similar levels to wild-type parasites and, importantly, at the correct stage of development.
A new complemented (knock-in, Kin) LmcDNA16 line was generated as described in Fig. 2, by introduction of a linear fragment containing the complete LmcDNA16 locus plus constitutively expressed NEO gene into its original location on chromosome 23 in a heterozygous (LmcDNA16 locus single deletion) background, using homologous recombination as described in McKean et al. (2001).
Before proceeding to sand fly transmission experiments therefore we generated a new complemented line expressing a single copy of the LmcDNA16 locus, coupled with a constitutively expressed NEO gene reintroduced into one allele of the original locus, thereby generating a heterozygous add-back parasite line.
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This process yielded a platform win-win: Both user experience and process efficiency improved, while new business capabilities complemented new economic efficiencies.
It predicts, in the form of the techniquark complexes, a whole new complement of massive particles.
Huber-Lang, M. et al. Generation of C5a in the absence of C3: a new complement activation pathway.
A new complement of court officers took charge, paunchy weekend cops who had oversize highway patrol sunglasses and gonzo equipment belts.
For more information about accessing your digital subscription benefits visit economist.com/digitalWe have put a lot of effort into developing this new complement to the weekly edition.
In the future as more and new complement inhibitors are being discovered, there is the possibility that a better inhibitor of complement activation will be found which will help provide a formidable treatment for autoimmune disease.
Seventy-eight million baby boomers are poised to eventually retire, to be replaced by a new complement of workers reflecting the richness and diversity of America.
By 1945, Texas was carrying 1,723 officers and enlisted men with the addition of crews for additional weapons as well as a new complement of U.S. Marines.
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