We cannot explain why 1 WCBV-neutralizing sample additionally neutralized DUVV.
At the end of neutralization, the sample was mixed, and diluted 1 10 with neutralizing broth.
Within these locations, flavivirus-specific NT-Ab responses were differentiated in several samples: neutralizing antibodies to either WNV or BAGV were detected in samples from 6 locations, whereas samples with neutralizing antibodies to either WNV or USUV were detected in 1 location.
Among 76 serum samples with WCBV-neutralizing activity, only 1 sample additionally neutralized DUVV, but no cross-neutralization to other lyssaviruses was detected.
However, the assay used in that study measured antibody concentration qualitatively (% neutralizing activity) and samples were taken 1 7 days after hatch [31].
A cell-based bioassay was used to detect neutralizing antibodies in samples that were positive in the immunoassay.
Several attempts to test the neutralizing activity of fecal samples failed each time, as the samples from the mice, including control, and even at high dilutions, were toxic for cell cultures an observation made by others as well.
Cytotoxicity prevented determination of neutralizing antibodies in 1 sample, which was therefore excluded from the analysis.
At 1/100 dilution, all samples had potent neutralizing activities capable of neutralizing at least 60% of input virion, with median level activities larger than 95% (Fig. 2C).
Most of the samples contained neutralizing antibody at a titer of 40 or 80 (Table), though no samples produced a 4-fold titer change that would suggest that TAHV infection caused the initial illness.
In another study of Nigerian fruit bats, 2 of 50 serum samples had neutralizing antibodies against CVS-11 but failed to neutralize DUVV (20 ).
