This is exemplified by antibodies neutralizing heat-labile toxin (LT), which is secreted by enterotoxigenic E. coli, and the closely related cholera toxin (CT), produced by Vibrio cholerae.
The neutralizing activity of heat-inactivated sera (56°C, 30 min) was determined by mixing 10 ng of pseudotype virus (in 30 µl) with diluted serum (in 30 µl) at 37°C for 1 h.
Parainfluenza virus 5 (PIV5) and human parainfluenza virus type 2 were effectively neutralized in vitro by naïve AGM sera, but neutralizing capacity was lost by heat-inactivation.
A seroneutralization assay was performed by using baby hamster kidney cells to screen for neutralizing antibodies against CHIKV in heat-inactivated monkey serum samples.
When CaCO3 is used as a neutralizing agent instead of Na2CO3, additional heating after the fermentation was supposed to be required to dissolve calcium fumarate and the excess of CaCO3 that usually stick to the cells.
injection of lung carcinoma cells induces hyperalgesia in mice accompanied by high peripheral production of IL-1 β and TNF α, and the treatment with etanercept (a TNF-neutralizing soluble receptor) prevented the development of heat hyperalgesia.
In order to investigate the neutralizing activity of induced polyclonal antibodies, antisera were heat-inactivated at 56°C for 30 min and then incubated with standard BAFF.
Immune sera from orally inoculated mice were found the most potent to neutralize in vitro dengue infection with neutralizing antibody activities in some cases higher than that obtained with the immune sera from mice i.p. injected with heat-inactivated virus.
Samples were heat inactivated at 56°C for 30 min and tested for neutralizing antibodies at a 1 10 dilution against WNV strain NY99 4132, originally isolated from the brain of a dead crow in New York, and St . Louisencephalitis virus strain TBH-28, originally isolated from a person in Florida, USA.
Serum samples were inactivated by heating at 56°C for 30 min, and an orthopoxvirus plaque-reduction neutralizing test (PRNT) was performed.
Heat-denatured CCL2-neutralizing antibody was, however, ineffective at preventing THP-1 monocyte chemotaxis in response to TNFα-conditioned media.
