Gels were denatured for 45 min in denaturation solution (0.5 M NaOH; 1.5 M NaCl), neutralized for 45 min in neutralization solution (1 M Tris, pH 8.0; 1.5 M NaCl), and dried at RT (45 min) and 60°C (45 min).
After each exposure time, 2 mL of the corresponding neutralizing agents were added to the test tubes and neutralized for 10 min.
After the appropriate contact time, 2 mL of the corresponding neutralizing agents were added to the test tubes and neutralized for 10 min and viable bacteria counts were monitored.
Membranes were kept for 4 min onto a Whatmann 3 MM paper saturated with denaturation buffer (0.5 M NaOH, 1.5N NaCl), treated for 10 min at 100°C, and neutralized for 10 min on Whatmann 3 MM paper saturated with neutralization buffer (1.5 M Tris-HCL pH7.4, 1.5N NaCl).
The drivers managed to slow down, and the race was neutralized for several laps before restarting on Lap 40.
With this election, Mr. Khamenei and his protégé appear to have neutralized for now the reform forces that they saw as a threat to their power, political analysts said.
For the conditions that all the carboxylic groups in BCA molecules were totally neutralized for the four kinds of BCAs, Zr-TMSA possessed the highest activity (Entries 1, 5, 7, 9).
While it is common knowledge that ion beams are easily neutralized for both current and charge density using a variety of means, the precise process of neutralization remains unknown.
The emissions due to heating and cooling are neutralized for the occupancy phase and only those of the building's embodied energy, demolition and maintenance phases are taken into consideration.
Now, in response one could claim that once the question of whether the animals are disguised mules has been raised, the evidence is "effectively neutralized" for both the former and the latter, and S is no longer justified in believing that the animals are zebras.
