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The eluted samples were neutralized immediately with a 30 μl of neutralizing buffer (1 M Tris-HCl, 2 M NaCl, pH 8.8).
Eluted fractions were neutralized immediately with 0.1 M Tris (pH 9, 100 μl/ml elution buffer).
As a result, the friction surfaces carry opposite electrostatic charges which will not bleed off or be neutralized immediately since both the polymer films and air are insulative.
"We need to address the subject of treating terrorists after they get neutralized immediately.
The eluted fractions were neutralized immediately with 10 mM PB (pH 7.4), pooled, and dialyzed.
The eluted fractions were neutralized immediately with 1 M Tris-HCl (pH 9.0) [17], pooled, and dialyzed.
Similar(51)
Fractions containing pure complex were pooled, neutralized and lyophilized immediately to prevent complex dissociation.
The bound phages were retrieved by adding 0.2 M Glycine HCl (pH2.2) containing 0.5 % BSA, and immediately neutralized with 1 M Tris HCl (pH9.1).
The nitrogen ions and silver particles arriving at the exposed silicon substrate were immediately neutralized since the silicon substrate was conductive.
OH− and TPP ions in the acidic CS solution could compete to interact with the -NH3+ of CS, but OH− ions would be immediately neutralized by H+ ions and increase the pH of the CS solution.
After blocking with 0.1% casein in phosphate-buffered saline (PBS) for 2 h and incubation with phage-displayed sdAbs in PBS for 1 h at room temperature, the specific phages were eluted with 100 mM triethylamine, transferred to a fresh tube, and immediately neutralized with 1.0 M Tris-HCl (pH 7.4) and used to infect TG1 cells.
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CEO of Professional Science Editing for Scientists @ prosciediting.com