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Gels were denatured for 45 min in denaturation solution (0.5 M NaOH; 1.5 M NaCl), neutralized for 45 min in neutralization solution (1 M Tris, pH 8.0; 1.5 M NaCl), and dried at RT (45 min) and 60°C (45 min).
After each exposure time, 2 mL of the corresponding neutralizing agents were added to the test tubes and neutralized for 10 min.
After the appropriate contact time, 2 mL of the corresponding neutralizing agents were added to the test tubes and neutralized for 10 min and viable bacteria counts were monitored.
Membranes were kept for 4 min onto a Whatmann 3 MM paper saturated with denaturation buffer (0.5 M NaOH, 1.5N NaCl), treated for 10 min at 100°C, and neutralized for 10 min on Whatmann 3 MM paper saturated with neutralization buffer (1.5 M Tris-HCL pH7.4, 1.5N NaCl).
The drivers managed to slow down, and the race was neutralized for several laps before restarting on Lap 40.
With this election, Mr. Khamenei and his protégé appear to have neutralized for now the reform forces that they saw as a threat to their power, political analysts said.
Similar(27)
We also offer the pictures of MLR with or without neutralizing for IFN-α in order to show the states of cell growth.
Instead, an explanation was found in the changes of pH [[11]] or ion distribution/hydration near the dispersed particles to form neutralized surfaces for magnetically enhanced coagulation [[12 14]].
After the time of study, the samples were neutralized except for thermal-, UV-, and peroxide-treated samples and diluted with methanol.
Some terminal ileum sample was fixed in 10% neutralized formalin for immunohistochemical staining.
Tumor tissue was fixed in 4% buffered neutralized formalin for 48 hours.
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