Exact(1)
A neutralizer assay was conducted according to ASTM E 1054-08 [ 13] demonstrating that test products were effectively neutralized (data not shown).
Similar(58)
It did not neutralize virions (data not shown).
We then obtained an indication towards contamination with type I IFN from competition studies showing that the contaminant in mAb preparations was neutralized by rabbit (data not shown) or sheep polyclonal anti-human IFN-α antiserum (Fig. 1D).
Surviving AID−/− had high poxvirus specific neutralizing IgM titers (data not shown).
However, there was a minor effect by monovalent RSV-E4, which did not neutralize the RSV Long strain (data not shown).
Although Ad-gBCMVpoly immunized mice showed better protection against Vacc.gB when compared to Vacc.IE-1 (Figure 6A), this better protection was not due to anti-gB antibodies (Figure 6B) as Vacc.gB was not neutralized by serum from immunized animals (data not shown), but due to gB-specific CD4+ T cell responses (Figure 6C).
Test products were effectively neutralized using ASTM E 1054-08 as described above (data not shown).
Infection with the VSV-pseudotype was neutralized with rabbit serum to REBOV-GP (data not shown).
Removing gp150-specific antibodies from immune sera had no effect on the neutralization of BHK-21 cell infection (data not shown) and pooled gp150 knockout-immune sera neutralized wild-type MHV-68 for BHK-21 cell infection somewhat better than pooled wild-type-immune sera did (Fig. 5A), consistent with gp150 not being a significant neutralization target.
One antibody clone bound to mouse TNF-alpha with a high affinity (Kd = 1.4 nM, measured by Surface Plasmon Resonance on a Biacore3000, Fig. 3A), and good specificity (the epitope mapped to amino acids 19 40 of the TNF-alpha mature chain, data not shown), and could neutralize mouse TNF-alpha activity by blocking TNF-alpha's cytotoxic effect on L929 cells (Fig. 3B).
Moreover, in a subset of enabler resistant cell lines only putative activator BH3 peptides induced cytochrome c release, whereas combinations of enablers that similarly neutralize all pro-survival members remained ineffectual (data not shown).
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