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Epitope masking is expected to be the major factor reducing the practical cross-strain neutralization value of any given conserved anti-variable-loop epitope from its theoretical maximal cross-strain reactivity to its practical cross-strain reactivity expected for a vaccine.
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GraphPad Prism 4 for Windows (www.graphpad.com/welcome.htm) and nonlinear regression analysis were used to calculate 50% neutralization values.
All animals in group 1, and animals 7, 13 and 14 showed significant neutralization titers (value of PRN70>1.2) after the first vaccine administration.
For neutralization, a standard value of 1,000 IU for 07/150 would be equivalent to a neutralization GMT of 518.
Two major techniques are currently used to estimate rabies virus antibody values: neutralization assays, such as the rapid fluorescent focus inhibition test (RFFIT), and enzyme-linked immunosorbent assays (ELISAs).
‡VN, virus neutralization test (ND100 log2); values >4 are considered positive.
When the charge density of micelle approaches a critical value as neutralization progresses, counterion condensation of Na+ ions on the polymer chains occurs.
In the vaccinated chickens, the titers of neutralization antibody reached 7 9 values, showing that protection could be explained by the induction of a sufficient humoral response.
For fractions I/2 and I/4 it was not possible to determine IC50 values because neutralization of 50% NO radicals was not accomplished even with the highest concentrations of 22 μg/mL and 120 μg/mL, respectively.
Interestingly, measurements of E. coli cells treated with increasing GO concentrations established a correlation between zeta potential neutralization and bioluminescence inhibition values at 60 min (r = 0.973, P < 0.01).
Although the ELISA appears simpler, safer and more efficient, the assay is less sensitive in detecting low values of rabies virus neutralizing antibodies than neutralization tests.
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