Exact(28)
The lysed liquid was neutralized with 5 µl neutralization solution(1 M HCl∶1 M Tris-HCl = 2∶3, pH 0.6).
Gels were denatured for 45 min in denaturation solution (0.5 M NaOH; 1.5 M NaCl), neutralized for 45 min in neutralization solution (1 M Tris, pH 8.0; 1.5 M NaCl), and dried at RT (45 min) and 60°C (45 min).
Neutralize by addition of 15 ml neutralization solution to return the pH to normal and allow the 2 strands of plasmid DNA to return to the native state.
At least they got to use the bomb squad's "mechanical" neutralization solution.
A critical step in plasmid purification is the use of the appropriate neutralization solution.
After incubation on ice for 10 min, an equal volume of neutralization solution (400 mM HCl, 600 mM Tris HCl) was added.
Similar(32)
Surface neutralization solutions (SNS) (patent pending) and/or the addition of calcium ions reduce the solubility of hydroxyapatite thus extending the column lifetime.
This type of reaction is called neutralization, because the solution is made neutral.
In our investigations we employed dialysis against deionised water as a method of neutralization of collagen solution.
The neutralization with supersaturated solution of SB, followed or not by ultrasonic cleaning results in lower mechanical strength of a pressable lithium disilicate ceramic.
Thus, it was concluded that neutralization with supersaturated solution of sodium bicarbonate, followed or not by ultrasonic cleaning results in lower mechanical strength of a pressable lithium disilicate ceramic.
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