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The 90%% neutralization sample titer was determined to be the sample dilution which resulted in 90%% neutralization of the Ad-luciferase vector replication as tested on A549 cells.
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At the end of neutralization, the sample was mixed, and diluted 1 10 with neutralizing broth.
To minimize inter-assay variations of neutralization activity, sample IC50s were normalized using B4 IC50 values obtained in each assay.
Data on neutralization of samples (for basic or acid substances).
To derivatize the carbonyl groups on the precipitated ATP synthase beta-subunit with DNPH, 10 µl supernatant solutions were incubated with 10 µl of 10 mM DNPH solution (OxyBlot kit, Chemicon) or 10 µl of control solution without DNPH for 15 min. After neutralization, the samples were subjected to the western blot analysis with rabbit polyclonal antibody against DNP-KLH (Invitrogen).
After AHP pretreatment and neutralization, biomass samples were typically lyophilized.
The diagnostic yield of the string test in this study is lower than previous studies [ 24]; it is important to note our lack of neutralization of the sample before storage as well as delays in processing samples may have decreased string test yield and is a limitation of our study.
The system was operated at a base pressure 4 × 10−8 Pa, the sample neutralization was performed with low energy electrons (<5 eV).
The system was operated at a base pressure of 4 × 10−8 Pa and the sample neutralization was performed with low energy electrons (<5 eV).
The system was operated at a base pressure of 4 × 10−8 Pa, the sample neutralization was performed with low energy electrons (<5 eV).
In a previous work having the purpose of knowing the reaction rate of the neutralization reaction, a sample of Nafion® 117 PFSA was immersed in a solution of 70 mL of distilled water and a specific amount of sodium or potassium hydroxide of known concentration.
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