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Both the virus and the reagent used for neutralization influence the assay sensitivity.
These data highlight that pro-inflammatory cytokines and their specific neutralization influence the expression of cellular receptors implicated in atherosclerosis.
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The aim of this study was to determine whether the validity of neutralization was influenced by the type of test (suspension test versus carrier test), by the type of microorganism or by different storage times of the neutralized antiseptic solution containing the particular microorganism.
Moreover, the high injection rate leads to neutralization of the influence of heterogeneity through the averaging effect of intense fracturing.
Another probable means for the local temporary neutralization of the influence of atmospheric oxidizers during ball lightning formation is the accidental simultaneous process of the generation of a reducing atmosphere of additional gas reductants—hydrogen, carbon monoxide, hydrogen sulphide etc.
Although individual sites usually minimally affect the global integrity of the glycan shield, we identify examples of how deleting some glycans can subtly influence neutralization by bNAbs that bind at distant sites.
Other factors influence neutralization even when the epitope is present and not masked.
CXCR2 neutralization did not influence JHMV viral loads within either the brain or spinal cord during chronic infection, as determined by measuring viral titers by plaque assay at defined times post-treatment (Figure 2B).
Consistent with low IL-22R expression in the spleen, IL-22 neutralization did not influence virus replication in this organ.
We were able to show for the first time that the validation of neutralization is strongly influenced by the amount of active agents, the amount of neutralizing agents, the storage time of the neutralized antiseptic before plating and by the chosen microorganism.
In summary, with the use of our R5 SHIV-C constructs in RM, SHIV-1157ipEL-p, SHIV-1157ipd3N4 and related viruses, we have shown that natural evolution of Env sequences was predominantly associated with changes in the V2 loops, which influenced neutralization sensitivity profiles.
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