Much research into protective, adaptive immunity against bacterial pathogens has focused largely on the role of neutralising antibodies.
After that slides were neutralised using neutralising buffer and stained with EtBr.
While the C-terminal repeat regions played the principal role in generating neutralising antibodies to TcdA, in the case of TcdB, the central region domains dominated the neutralising immune response.
Importantly, the endothelial cell growth inhibiton promoted by human cancer cell lines was reversed by anti-VEGF165b neutralising antibodies, indicating a major role for this factor as an orlistat-induced gene product.
We confirmed IL-17R-mediated modulation of synoviolin by the use of neutralising Abs against IL-17RA or IL-17RC [23].
Conformational changes in epitopic regions undoubtedly play an important – perhaps dominant – role in inhibiting binding by neutralising antibodies.
Mouse studies have shown that Gas6 is a platelet response amplifier that plays a significant role in pathological thrombosis, and that Gas6-neutralising antibodies inhibit platelet aggregation in vitro (Angelillo-Scherrer et al, 2001).
Further evidence supporting an antiapoptotic role for HIF-1α is demonstrated by the finding that a neutralising monoclonal antibody against vascular endothelial growth factor (VEGF), the major transcriptional target of HIF-1α, blocks the antiapoptotic effects of hypoxia in HepG2 cells [ 25].
