The chemical treatment of industrial effluent, containing sulphuric acid, is typically completed by neutralising it with lime through solid/liquid separation.
The uncle was suspicious that the gunpowder was bewitched, tried to neutralise it with fire, and in the resulting explosion was killed.
He developed a malaria treatment later sold commercially as "Livingstone's Rousers" The uncle was suspicious that the gunpowder was bewitched, tried to neutralise it with fire, and in the resulting explosion was killed.
Finally, as light beers proliferated, Anheuser-Busch clambered laboriously on to the bandwagon itself, with Natural Light and then Bud Light.Although these imitators eventually neutralised it, Miller's initial success with Lite fired marketers' imaginations: "dry" beer (no aftertaste), "ice" beer (frozen, filtered and thawed to extract water), and "clear" beer (why not?) all had their brief days.
As TCA also inhibits the downstream assay, it was neutralised with Tris-acetate (pH 7.75) to bring the total TCA percentage to 0.1% following the manufacturer's direction.
Then it was neutralised with 1 M HCl to pH 6 8, an equal volume of 0.1 M Tris-HCl buffer was added and the sample was heated for 30 min in a boiling bath.
The resulting clear red-brown solution was stirred for 0.5 h before it was diluted with H2O (10 mL), neutralised with 5 m NaOH and treated with 1 m NaSO3 (5 mL).
Samples were neutralised with acetic acid, mixed with scintillant and counted as above.
After 12 hours, the reaction was neutralised with hydrochloric acid and extracted with ethyl acetate (3 times).
The eluate was neutralised with ammonium acetate and incubated with THP-mal-J591c-scFv at room temperature.
