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Membrane pieces were washed thrice with TBST and antibodies were eluted by adding 200 μl 100 mM Glycine-HCl (pH 2.5) and vortexing for 1 min. Purified antibodies were then transferred to fresh tubes and neutralised immediately by adding 10 μl 1 M Tris-Base.
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The membrane was washed extensively in PBS and the antibody was eluted from the membrane in 200 mM glycine pH 2.5 and immediately neutralised.
The fractions were immediately neutralised and submitted to SDS PAGE and western blotted with TKH2.
The elution fraction was immediately neutralised with 2 M Tris buffer.
The protein eluate was obtained with 50 m M glycine pH 3.0, and immediately neutralised with 1/10 volume of 1 M Tris-HCl, pH 8.0.
After each selection, the IGFBP7-specific phages were eluted with 100 m triethylamine (pH 10.0) and immediately neutralised with 1 Tris-HCl, pH 7.5.
After washing step, the specifically bound phages were eluted with 0.1 M Glycine-HCl, pH 2.0 and immediately neutralised with 2 M Tris.
Allergen-Fab fragment complexes were eluted with 0.1 M glycine-HCl (pH 2.0) and immediately neutralised with 1 M Tris buffer.
The supernatant fractions were immediately neutralised and determination of adenine nucleotides was performed by high-performance liquid chromatography, as previously described [ 18].
The protein eluate was obtained with 50 m glycine pH 3.0, and immediately neutralised with 1/10 volume of 1 Tris HCl, pH 8.0.
The Fed is obviously worried about runaway inflation, but given economic weakness, it seems odd to want to immediately neutralise any potential use of the funds it has provided to banks.The thing is, Ben Bernanke is not unaware of the potential good that could come from inflation.
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