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Polyclonal antibodies were eluted from the columns with Gly/HCl buffer (pH 2.40), followed by immediate neutralisation to pH 7.5.
We demonstrate the ability of COX-2 inhibition and AR neutralisation to inhibit HCA7 cell proliferation with an additive effect seen with a combination of both.
There was also a trend for TNFα neutralisation to diminish the stimulatory effects of treated osteoclast-derived conditioned medium but this was not statistically significant versus the isotype control.
Briefly, concentrated basic sodium silicate (Aldrich Chemical Co., Poole, UK) was diluted in 2.5 L ultra-high purity water, followed by pH neutralisation to 7.2 with 5 mol/L hydrochloric acid (volumetric standard; Aldrich Chemical Co).
F11 cells (A gift from T. Wyss-Coray) seeded at 4.10 × 10 cells/well in 96-well flat-bottom tissue culture plates were incubated with 50 μL activated homogenised lung sample (activated by addition of 2.5 μL 6 m HCl for 10 min followed by neutralisation to pH 7.4 with 6 m NaOH) for 24 h.
sgp130 was given 30 min prior to the start of laser treatment and 3 h after ending PDT treatment, at a time point when we expected an increase of local IL-6 and sIL-6R α and its neutralisation to be optimal.
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Elution was performed with 0.1 M glycine/HCl buffer pH 2.4, followed by re-neutralisation to pH 7.4.
Alkaline denaturation was performed by addition of 0.4 M NaOH to 0.32 M (~ 240 μl added to 54 μl of sample), and incubation at room temperature for 10 minutes. 1 M Tris Hcl (pH 8) was added to 0.02 M prior to neutralisation (pH 8 to 8.5) with 0.4 M HCl.
Neutralisation refers to an attempt to reduce, remove or compensate for the discomforting effect of an intrusive thought, image or impulse (Rachman, Shafran, Mitchell, Trant, & Teachman, 1996).
To date there are over 60 types of adenoviruses identified [ 9], grouped into seven species A to G on the basis of their resistance to neutralisation by antisera to other known human adenoviruses [ 10, 11] or genome analysis [ 11].
In a previous study, the immunity status of 10 members of the laboratory staff with OPV history against CHAT was analysed using neutralisation test to estimate the potential risk of laboratory-associated infections and transmission to the community.
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