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Neutralisation time: 1, 2, and 4 hours.
The percentage of FCS was therefore fixed at 3%, with a neutralisation time of 1 2 hours for the final protocol.
Percentage of FCS (from 1-51-5%neutralisation time (1 4 hours), age of cells (3 to 16 days), and different batches of FCS gave the same result and were not crucial within the tested range.
Similar(57)
As expected, the placebo-dosed patients showed no target neutralisation across all times points.
The slides were then washed 3 times with neutralisation buffer [0.4 M Tris (pH 7.4)] for 5 min each.
Because 43RCA neutralisation activity was four times higher than 38RCA, we focused our work upon this more efficient scFv.
Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs) which are time-consuming and require BSL3 facilities.
Future work may optimise bleach-sedimentation by modifying bleach concentrations, sedimentation times, improving bleach neutralisation, by processing larger sputum volumes or by using filtration [ 24].
At all detection time points, non-specific neutralisation antibody titres in the mock group were lower or equal to 1 10.
With time, this is inhibited by neutralisation of the macrophage-produced, proinflammatory cytokines driving MMP production, but by then, large quantities of these MMPs have already been produced, thus blurring the results.
At present, the recommended neutralisation tests (FAVN test or RFFIT) are time-consuming, expensive and require highly trained technicians as well as special laboratory facilities.
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