Sentence examples for neutralisation solution from inspiring English sources
Samples were derivatised, incubated at room temperature, and neutralisation solution added.
HCl +10 ml methanol +38 ml H2O) for 15 min at 50°C with intermittent gentle shaking, before decanting and re-suspending in a neutralisation solution (7% NaOH in 60% ethanol) for 15 min at RT. Solutions were discarded and retained samples were rehydrated using a series of decreasing concentrations of ethanol (from 40, 20 and 10% v/v).
Then the migrated cells adhering to the underside of the membrane and from the lower chamber were dislodged by incubating the inserts in 0.25% trypsin for 4 min followed by treatment with trypsin neutralisation solution (Promocell).
Samples were denatured with 6% SDS incubated with the provided DNPH solution to derivatise any carbonyl groups for 15 min, and the reaction was stopped with the provided neutralisation solution.
In practical terms, the following samples are processed in parallel: T samples are representative of the total amount of DNA present and are obtained by adding neutralisation solution prior to the alkaline unwinding solution.
DNA from pulsed-field gels was transferred to a nylon membrane (Roche) as previously described [ 60] with the following modifications: 2 × 20 min incubations in denaturation solution, 2 × 20 min incubations in neutralisation solution and a 48 h transfer.
Immunoprecipitated C4.4A was eluted from the protein G-antibody complex using 100 m M glycine at pH 2. After neutralisation, the solution was adjusted to 2% Triton X-114 and incubated for 1 h at 4°C.
Thanks to a new experimental bench, the influence on the oxidation rate of seven treatment parameters is investigated (current density, time of treatment, acid concentration, mass of powder, particles size, stirring, neutralisation by ammonia solution).
Sodium hydroxide was added until neutralisation of the solution.
Neutralisation of the treated solution with a reducing agent (1 mL of 25%w/v sodium thiosulfate solution per 4 mL of bleach) is required before disposal [ 60].
Energy escalation related to combustion, solution, and neutralisation has been presented, along with pyrophoric scenarios.
