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Pretreatment with NF-κB inhibitor significantly reduced NF-κB expression and the number of intact motor neurons when compared to the control group.
These results again suggest higher degradation of syntaxin1B in activated CHL1−/− neurons when compared to CHL1+/+ neurons.
Statistical analyses revealed a significant decrease of neuritic length (−25%) in ExpHtt expressing neurons when compared to Htt-transfected cells.
This technique also showed alpha-synuclein accumulation in DMV neurons when compared to controls (Figure 3D F).
Stereologic cell counts in control animals showed a 63% survival rate of TH positive neurons when compared to the contralateral side.
As expected, total dendritic length was severely decreased in cul1 mutants and increased in cul3-mutant ddaC neurons, when compared to wild type (Figure 5B).
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Neurons transfected with GFP-N-WASP (Figure 7C) showed a prominent increase in the number of neurites extending from each neuron when compared to control cells transfected with GFP alone (Figure 7A).
Furthermore, recent evidence from transgenic mice suggests that direct expression of GDNF by astrocytes within the spinal cord does not result in motor neuron protection when compared to muscle delivery [15].
Their experiments (soon to be published) have demonstrated the superior ability of this line to silence neuron activity when compared to TeTx.
In the DMV, 1.5 and 3 months rotenone treatment resulted in increased intracellular alpha-synuclein accumulation inside ChAT+ neurons (Figure 3B) when compared to controls (Figure 3A).
Interestingly, we observed only a moderate downregulation (14 probesets with 8>1.5 fold), but a striking upregulation of genes (92 probesets, 63>1.5 fold) in normal control neurons from males when compared to females (mN_fN) and the majority of these genes were downregulated in fPD (Figure 2A).
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