Sentence examples for neurons were washed from inspiring English sources

Toxins were then incubated with cultured rat hippocampal neurons for 5 min. Neurons were washed and incubated in neuronal media at 37 °C for 20 h.

Neurons were washed twice and protein were precipitated with trichloroacetic acid (TCA; 10%, w/v).

Immediately following stimulation, neurons were washed with ice-cold PBS and then lysed with 215 µL ice-cold lysis buffer.

Neurons were washed and then stimulated with 0 or 25 µM NMDA/TTX for 3 min, and then washed and further incubated for the indicated durations.

Neurons were washed with PBS, incubated for 60 minutes with secondary antibodies and DAPI at room temperature, washed with PBS, and mounted with Gel/Mount.

Neurons were washed with PBS/30 mM glycine pH 2.5 to remove the remaining surface label, fixed, and immunolabeled using antibodies against Tmub1/HOPS or VAMP2.

Hippocampal neurons (DIV7) were washed twice with ice-cold PBS and once more with PBS buffer supplemented with 1 mM DTT and a cocktail of protease inhibitors (0.1 mM PMSF; CLAP: 1 µg/ml chymostatin, 1 µg/ml leupeptin, 1 µg/ml antipain, 1 µg/ml pepstatin; Sigma-Aldrich Química, Sintra, Portugal).

To preload SCG neurons, dishes were washed with KRH assay buffer (as above) containing 100 nM raclopride.

For estimating the number of synaptic boutons in autaptic neurons, cells were washed in PBS, fixed with 4% paraformaldehyde in PBS, permeabilized and blocked in 0.2% Triton X-100/PBS with 10% blocking serum, and incubated at 4°C overnight with mouse monoclonal synapsin1 antibody (1 1000 SynapticSystems, Goettingen, Germany) diluted in 1% blocking serum/PBS.

18 Briefly, neuron cultures were washed with phosphate-buffered saline (PBS -based rinsing buffer and incubated for 30 minutes at 4°C with 1mg/ml Sulfo-NHS-Biotin (Thermo Fisher Scientific, Waltham, MA) in rinsing buffer.

After 3 d, the EngNT-neuron cocultures were washed briefly in PBS and fixed in 4% PFA at 4°C for 24 h, followed by immunofluorescence staining as described previously for collagen gels (36, 37), to detect β-III-tubulin positive neurons and S100-positive Schwann cells.