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Cerebellar granule neurons were homogenized in 50 μl 50 mM MOPS pH 7.4, 250 mM sucrose, 1 mM EDTA, 0.1% ethanol (v/v) and 10% protease inhibitor mix M (Serva, homogenization medium) using a Potter-Elvehjem homogenizer (2-ml size).
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To extract whole cell, cytosolic and mitochondrial fractions, cells or neurons were homogenized and mitochondria pelleted as previously described.
Tissues were homogenized in tissue homogenizer.
Osteoclasts were homogenized in a hand homogenizer with 20 25 strokes.
For the preparation of total lysates from cultured astroglia and neurons from WT or Cln1 −/− mice, cells were homogenized in Phosphorate extraction reagent (EMD Biosciences, Billerica, MA).
Retinas were homogenized in T-PER using a glass homogenizer.
Three d later, the neurons were subjected to OGD treatment with or without bafilomycin A1 (100 nM ., Mouse non-ischemic and ischemic brain tissues were homogenized in PBS with a protease inhibitor cocktail.
Two-week-old rice seedlings were homogenized in liquid nitrogen.
Both kinds of material were homogenized in liquid nitrogen.
Samples were homogenized in 0.03 N HCl.
Whole lung tissues were homogenized in hypotonic buffer.
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