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During the second week, significantly more neurons were growing on NMD compared to CSF (59% neuronal in NDM vs. 24.0% neuronal in CSF, P<0.005) and thus more glial cells were growing in CSF than in NDM (41% glial in NDM vs. 76% glial in CSF).
In the presence of Cntn4, -5 or -6, all neurons were growing concomitantly in length and branching as shown by plotting the number of roots versus the number of segments (supplementary material Fig. S1).
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Dissociated neuronal cultures from neonatal hippocampal neurons were grown [20] on 60-channel multielectrode arrays (MEA) (Figure 1B, left, see Methods) with 50 to 55 active sites.
The neurons were grown and differentiated on the hydrophilic functionalized CNT microelectrodes, and they were repeatedly excited with charge-unbalanced stimulation protocols.
In comparison with a standard sample (i.e., cultured neurons on a PLL-coated silica substrate), hippocampal neurons were grown similar to a standard one with many protruding processes, except for the case of ZnO nanowires.
TSM1 and primary cultures of neurons were grown on polylysine-coated glass coverslips.
Neurons were grown for at least two weeks in culture to allow maturation of glutamatergic synapses [25].
Neurons were grown for one week on poly-L-lysine-coated 8-chamber slides in neurobasal medium with B27 supplement.
By contrast, the immunoreactive area occupied per neuron was significantly higher, by a mean of 138±5.2% (p<0.001), if neurons were grown on astrocytes transduced with Lv-shGFAP.
Neurons were grown in 24-well plates at 37°C in 5% CO2 and were grown for a total of 72 h.
The hippocampal neurons were grown in culture for 10 to 14 days on 13-mm-diameter coated glass coverslips in 24-well plates.
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