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Laminin pre-treatment had an even more profound effect after 14-days of differentiation (neuronal stage).
In the present study, 87% of the 262 DAS exons were found to change in the neuronal stage of P19 cells by semiquantitative RT-PCR (Table 2).
Moreover, Rest levels had to be reduced by more than 92% before an increase in cell death at the neuronal stage was observed.
However, at the neuronal stage, there was markedly more cell death in REST-null cells than those in the control and REST/KD-50 cells (Fig. 4F 4H), which is correlated with the ailing look of neurons derived from REST-null ES cells (Fig. 3H and 3I).
Masking at the neuronal stage therefore manifests as almost a pure response gain change in this phenotype.
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The APP protein, among its non-pathological functions, is active in the "neuronal pruning" stage of brain development, wherein neurons in the early brain are selectively removed after rapid proliferation [ 111].
The first three stages of neuronal differentiation, with stage I being defined as the lamellipodia stage, stage II as outgrowth of neuritic process, and stage III as axonal outgrowth, occur very rapidly within 36 h of in vitro culture, while stage IV, during which dendritic growth is triggered, begins 2 3 days later, with their subsequent maturation (stage V) occurring beyond 1 week of culture.
Antibodies against enolase, Tuj1, NF, and GFAP were used to evaluate the neuronal differentiation stage.
These analyses suggest that the cluster C and D tumors express a set of genes marking a certain neuronal differentiation stage or lineage and have relatively low cell cycle activity.
This discrepancy may be attributable to the difference in neuronal developmental stage, that is, DIV.
The targeting of Rab17 to the dendrites is neuronal differentiation stage-dependent: at an early stage (3 d of in vitro culture) it localizes only in the cell body, whereas at a later stage (11 d of in vitro culture) some of the Rab17 is translocated from the cell body to the dendrites.
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